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Prof. LIU Wei's group published a new paper about mitotic Golgi fragmentation in Journal of Cell Science

来源 : admin     发布时间 :2013-05-15    浏览次数 :922

The latest issue of Journal of Cell Science published AMPK phosphorylates GBF1 for mitotic Golgi disassembly, the newest results of Prof. LIU Wei’s group in Molecular Research Center, School of Basic Medical Sciences (BMS).


In mammalian cells, the Golgi apparatus undergoes extensive fragmentation during mitosis; this is required not only for the partitioning of the complex but also for the process of mitosis. However, the molecular mechanism underlying the mitotic fragmentation of the Golgi is far from clear. Here, we show that AMP-activated protein kinase (AMPK) is phosphorylated and activated when cells enter mitosis. Activated AMPK phosphorylates GBF1, a guanine nucleotide exchange factor (GEF) for Arf-GTPases at Thr1337, disassociating GBF1 from the Golgi membrane and abolishing the action of GBF1 as an Arf1-GEF. We further demonstrate that the phosphorylation of AMPK and GBF1 is essential for Golgi disassembly and subsequent mitosis entry. These data suggest that AMPK-GBF1-Arf1 signaling is involved in the regulation of Golgi fragmentation during mitosis.


This project was completed by MAO Luna, an undergraduate student majoring in clinical medicine of Faculty of Medicine and LI Ning, a Ph.D student of BMS. The project was financially supported by the National Scientific Foundation and the 973 Project of China.